Automation.ini is a file that resides in the application folder of Spybot - Search & Destroy.
It currently is used in combination with the command line parameter /singleproductscan by specifying the single product to be scanned for in the following way:
Another use is in combination with /createresultsxml PT, which is another special automation option. In this case, you use it to specify a filename:
XMLLocation=<fully qualified filename>
Beginning with Spybot - Search & Destroy 1.6 RC 1, this also supports path templates.